DOT Drug Test in Ashburn, VA
DOT-compliant 5-panel urine drug screen for commercial drivers.
Walk-in price
DOT Drug Test
- Urine sample
- Same day for negatives
- Doctor’s order included
- HSA / FSA accepted
42775 Generation Drive, Ashburn, VA 20147
What this test screens for
Understanding the DOT Drug
A DOT drug test is the federally regulated 5-panel urine drug screen required for CDL drivers and other safety-sensitive roles in industries regulated by the US Department of Transportation. The panel screens for amphetamines, cocaine, opiates, phencyclidine (PCP), and THC (marijuana) using a strict federal collection and reporting protocol (49 CFR Part 40).
At our Ashburn lab, a DOT drug test is $79 and follows full chain-of-custody using the federal Custody and Control Form (CCF). Bring photo ID and any paperwork your employer or Designated Employer Representative (DER) has issued. Walk-ins are accepted Monday through Friday from 10 a.m. to 3 p.m. Negatives are typically reported the same business day; non-negatives are confirmed by GC/MS or LC/MS at the certified reference lab and return in 24 to 72 hours.
A Medical Review Officer (MRO) reviews any non-negative result before it is reported to the employer, reconciling against legitimate prescription medications the employee discloses. DOT-regulated testing differs from non-DOT employer testing in collection procedure, paperwork, and reporting chain — a 10-panel cannot substitute for a DOT 5-panel even though it covers the same substances.
Why people get this test
- CDL driver pre-employment screening (required before first dispatch)
- DOT random pool testing administered by your employer
- Post-accident testing per DOT regulations
- Reasonable-suspicion testing per DOT regulations
- Return-to-duty and follow-up testing after a previous violation
What to expect
From check-in to results — what your visit looks like
Most visits take under 15 minutes. Here’s the full arc, start to finish.
How to prepare
No fasting and no medication changes. Drink water normally so you can produce an adequate urine sample at check-in. Bring photo ID and any chain-of-custody paperwork your employer or DER has issued. Have a list of prescription medications available — the MRO will need it if any non-negative requires reconciliation.
During your visit
The collector verifies your ID and the CCF, then directs you to a private restroom following standard DOT collection procedures (no flushing, water turned off, blue toilet dye). You provide the sample, the collector measures temperature and volume, and the sample is sealed in front of you, signed off, and packaged for shipment to the certified reference lab.
Getting your results
Negatives are typically reported the same business day. Non-negatives are sent to confirmation testing — confirmed results return in 24 to 72 hours and are reviewed by an MRO before reporting to your DER. The CCF and the lab report together satisfy DOT documentation requirements.
Confidential
Your results never go through your insurance carrier.
Transparent
Price you see is price you pay.
Fast
Most results in 24–72 hours through our portal.
Doctor’s order
Included as part of every visit. No referral needed.
FAQ
DOT Drug Test — what people ask
Specific questions about the dot drug test. For anything not covered, call 571-500-7030.
Is a 10-panel drug test acceptable for DOT?
No. DOT requires the federal 5-panel collected and processed under DOT-specific chain-of-custody on the federal CCF. A 10-panel covers the same substances plus others, but cannot satisfy the DOT regulatory requirement.
What if I take a prescription that could trigger a positive?
Bring your prescription bottle or a pharmacy printout to the visit. Any non-negative result is reviewed by a Medical Review Officer who reconciles the result against documented prescriptions before reporting to your employer.
Do I need an appointment, or can I walk in?
Walk-ins are accepted Monday through Friday from 10 a.m. to 3 p.m. for employer-based drug testing. If your employer specified a window, follow their instructions; otherwise, arrive during open hours with your CCF and photo ID.
You might also need
Other drug & alcohol tests
Other tests our Ashburn neighbors commonly add to a visit.
5 Panel Drug Test (Urine)
Industry-standard 5-panel screen — amphetamines, cocaine, opiates, PCP, THC.
10 Panel Drug Test (Urine)
Screens for 10 common drugs of abuse including amphetamines, opiates, THC, cocaine and more.
12 Panel Drug Test (Urine)
Expands the 10-panel to add benzodiazepines and methadone — popular with healthcare and probation programs.
Before you walk in
Helpful guides
Quick, plain-English answers to the questions Ashburn neighbors ask most often before booking.
How long do drugs stay detectable? Detection windows by sample type
Urine, hair, and nail samples cover different detection windows. Pick the right sample for your situation.
Read the guide5 panel vs 10 panel drug test — what is the difference?
The 5-panel is the DOT standard. The 10-panel adds prescription medications. Pick based on your employer or court requirement.
Read the guideHow much does a drug test cost in Ashburn?
Real walk-in drug test prices, by panel and sample type. No insurance, no surprise bills.
Read the guideService area
We serve these communities
We serve Ashburn and the surrounding Loudoun and Fairfax County communities from our Goose Creek Village lab.
Book your dot drug test today.
Walk in or reserve a time online. Most visits take under 15 minutes.